attune flow cytometer - An Overview

attune flow cytometer - An Overview

Blog Article

Information-enriched characterization of cell populations. Morphological data from pictures can incorporate towards the richness of flow cytometry data. By way of example, the figure exhibits an if not standard apoptosis assay making use of Annexin V and PI, incorporating mobile imaging to characterize cells in Just about every population to reveal morphologically unique options. These insights could not are acquired from multiplex staining by yourself.

As samples are acquired around the Attune CytPix Flow Cytometer, the significant-pace brightfield digital camera captures and retailers photos of detected situations in a price approximately 6,000 photos for every next, based on the flow fee and graphic sizing. For greater overall flexibility, Attune Cytometric Software helps you to regulate the picture seize frequency as essential and choose a particular gate for impression capture, if essential.

Merge acoustic focusing know-how with significant-parameter Examination for adaptable spectral unmixing and conventional workflows.

Find out new insights into your experimental information by visualizing populations working with mixed fluorescence and morphology facts. Our software interface wizards empower downsampling, concatenation (merged information) and dimensionality instruments which can be created into your software package for an intuitive, streamlined information Evaluation working experience.

*Pricing is indicative only and is also topic to change. Make sure you Get in touch with your Agilent representative with any queries. The knowledge established forth herein will not be a suggestion or simply a determination, both equally of that happen to be subject to credit history acceptance, conditions and terms, and availability with Agilent. Agilent Economical Solutions is available in picked international locations.

Here, a skilled user has gated singlets confidently. Soon after evaluating the guide singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate is made up of a lot more than four% aggregates.

Optimizing gating approach. Even robust handbook singlet gating is error-prone and remains a subjective selection place in almost all flow cytometry assays. Imaging may be used to substantiate and modify gates to include only one cells of interest.

Dependable image good quality even at higher throughput Acoustic focusing as well as a higher-pace camera Mix to impression these T cells constantly at lower or large flow prices. Easily regulate focus and camera configurations read more to satisfy experimental needs.

Early signs of this contamination experienced at first been dismissed as debris. Backgating to discover new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also permits you to use morphological characteristics to find fascinating subpopulations that will not be apparent from flow cytometry knowledge by itself.

End users can perspective occasion photos in authentic-time to verify sample good quality, improve protocols, and record the morphology of each cell populace recognized for Evaluation. Imaging seize also Advantages from acoustic concentrating, which helps you to situation the cells in order that a pointy, centered graphic is obtained.

AI-pushed automatic graphic Examination Using the Attune Cytometric Software program interprets function characteristics into distinct morphology parameters which might be coupled with regular fluorescence and scatter parameters to expose remarkably reproducible inhabitants alterations and special subsets.

Software note: Automated picture Examination reduces person-to-user variability in flow cytometry gating techniques

Built-in dimensionality reduction resources enhanced resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to verify expected populace resolution in lysed full human blood. User can view functions from each cluster for visual affirmation of mobile phenotypes.

No wash/no lyse leukocyte analysis with imaging. Cells had been obtained from 24-hour-outdated blood by dilution in 1 mM EDTA ( Skilled UX/UI permits users to teach AI products for expanded applications. (A) The “Coach Model” aspect gives annotation tools and actual-time design coaching precision.

Gating on these prolonged parameters permits you to swiftly and correctly detect populations of curiosity and ensure gating strategy with little if any guide overview. The Attune software package gives access to state-of-the-artwork equipment learning analysis equipment in just an expertly intended consumer interface, making it exceptionally simple to operate.

The Attune Xenith cytometer is a significant-parameter spectral analyzer, furnishing both spectral unmixing and conventional flow abilities for extensive and flexible cell Evaluation.

The Attune CytPix Flow Cytometer lets end users to visualise samples all through acquisition, verify the precision of gating procedures, and assist benefits with dependable documentation for publication uses.

What gave the impression to be a homogeneous populace is actually additional assorted—an Perception that needs to be regarded as when interpreting benefits.